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100G passive optical network
Sporni, Alex ; Tomašov, Adrián (referee) ; Horváth, Tomáš (advisor)
This diploma thesis deals with the current state of development of passive optical networks, with the main emphasis on high-speed passive optical networks of 50 Gb/s and higher. The opening part of the thesis provides a theoretical introduction to optical access networks, their division, elements of passive optical networks and the subsequent analysis of the latest standards. As part of the implementation, simulation topologies for various simulation scenarios are designed in the VPIphotonics Transmission Maker tool. The proposed simulations are properly evaluated in the next chapter. The quality of the proposed simulation topology is assessed using bit/symbol error rate and in some cases in combination with an eye diagram (quality factor). The output of the work are simulation scenarios for various configurations and modulation formats of the proposed optical networks. The output of the work is also a laboratory task, which will be used for teaching purposes.
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Heat-shock proteins and plant tolerance against high temperature stress
Ott, Kristián ; Hála, Michal (advisor) ; Konrádová, Hana (referee)
In context with ongoing climate change, the average temperature is still rising. This is a problem mainly for agriculture and production of groceries, because plants generally have difficulties when coping with ambient temperature higher than 40 řC. If there would be continuing trend in decrease of global food production, it could cause problems with feeding still growing world population. Organisms in general developed many mechanisms of stress adaptation. One of these evolutionary adaptions to high temperature stress is production of heat shock proteins (HSPs). HSPs are very conserved family of proteins present in both prokaryotes and eukaryotes. This thesis summarizes present knowledge about the HSPs and their function (not only) during the high temperature stress in plants, but some of the information presented in this thesis were obtained also from other organisms. The thesis starts with general introduction to the high temperature stress and how plants can sense the ambient temperature. Next is general mechanism of induction of HSPs and their classification, structure, and mechanism of action. The possibility of future use of HSPs in agriculture and medicine is opened in last chapter.
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100G passive optical network
Sporni, Alex ; Tomašov, Adrián (referee) ; Horváth, Tomáš (advisor)
This diploma thesis deals with the current state of development of passive optical networks, with the main emphasis on high-speed passive optical networks of 50 Gb/s and higher. The opening part of the thesis provides a theoretical introduction to optical access networks, their division, elements of passive optical networks and the subsequent analysis of the latest standards. As part of the implementation, simulation topologies for various simulation scenarios are designed in the VPIphotonics Transmission Maker tool. The proposed simulations are properly evaluated in the next chapter. The quality of the proposed simulation topology is assessed using bit/symbol error rate and in some cases in combination with an eye diagram (quality factor). The output of the work are simulation scenarios for various configurations and modulation formats of the proposed optical networks. The output of the work is also a laboratory task, which will be used for teaching purposes.
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Potential physiological significance of thermal preconditioning
Ptáková, Alexandra ; Novotný, Jiří (advisor) ; Benák, Daniel (referee)
Short-term exposure of cells or whole body to mild hyperthermia, known as thermal pre- conditioning, is an experimental method used to provide cytoprotection against subsequent, otherwise harmful or even lethal, stress conditions. Thermal preconditioning is relatively easily feasible and could be used to prevent various health problems. The aim of this thesis is to sum- marize current knowledge about the impact of thermal preconditioning on cells and the whole organism. The possibilities of how to perform this method effectively are described here, toge- ther with the molecular mechanisms involved in the induction of cytoprotection. Some areas where thermal preconditioning could be used are also mentioned. Key words: thermal preconditioning, hyperthermia, cytoprotection, HSP, ischemia-reper- fusion injury
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Mitochondrial proteases and their role in biogenesis of mitochondria
Krump, Ondřej ; Stibůrek, Lukáš (advisor) ; Pecina, Petr (referee)
Mitochondria are organelles of endosymbiotic origin responsible for many cellular functions, including bioenergetics, biosynthesis and apoptosis. Regulated protein turnover is crucial for proper mitochondrial function. It is controlled by cellular proteolytic system, especially by its mitochondrial part. This mitochondrial proteolytic system is comprised of severeal groups of proteases. The best characterized AAA+ proteases constitute hollow oligomeric complexes, in which the proteolytic domains are localized. Access to these domains is dependent on unfolding - an energy-consuming process driven by ATP hydrolysis mediated by ATPase domains of AAA+ protein. The main function of AAA proteases is proteolytic degradation of proteins, a part of quality control system of mitochondrial proteins. AAA proteases are localized freely in mitochondrial matrix (Lon and ClpXP), or anchorred in the inner membrane (i-AAA and m-AA). Processing peptidases cleave off the mitochondrial targetting sequences of nuclearly encoded mitochondrial proteins. Oligopeptidases cleave peptides produced by processing and proteolytical degradation to single amino acids. Incorrect function of various components of mitochondrial proteolytical system is implicated in several diseases, including certain forms of hereditary spastic paraplegia...
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